We develop tools for high resolution light microscopy. Our first product, the NanoGrid, consists of an array of sub-wavelength sized holes that can be used for highly accurate alignment of multi-wavelength images and for high-resolution tracking of stage movement.
Miraloma Tech was founded by Thomas Schenkel. Thomas received a PhD in Physics from the Goethe University, Frankfurt. He has published 90+ articles and has 15+ years of experience in applied physics and nanoscience.
The NanoGrid consists of an array of sub-wavelength sized holes that can be used for the highly accurate alignment of multi-wavelength images and for high-resolution tracking of stage movement. The NanoGrid is an alternative to the use of fluorescent particles for alignment purposes and stage movement tracking. The holes in the NanoGrid are imaged using trans-illumination, hence there are no issues with photo-bleaching. Regular spacing of the holes in the NanoGrid guarantees that an accurate transform function can be obtained throughout the image. Since background is minimal, it is possible to achieve extremely high signal/noise ratios using short exposure times. Therefore, the position of the holes can be determined with extremely high precision using Gaussian fitting or other methodologies to locate diffraction limited objects.
Shown here is the overlay of two images obtained using a 100x oil immersion objective, LED-trans illumination, an image-splitting dichroic mirror, and two cameras, each imaging light at a specific wavelength range. Using this set of images, a transform function can be calculated that can register the two images with a precision better than ~2nm.
For more information and sales please contact:
Miraloma Tech, LLC
P.O. Box 460475
San Francisco, CA 94146
The following publications used hole slides for multi-color allignment:
James, J.R. and Vale, R.D. (2012) Biophysical mechanism of T-cell receptor triggering in a reconstituted system. Nature 487: 64-69. doi:10.1038/nature11220. See supplementary information page 10.
Mark A. Dewitt, Amy Y. Chang, Peter A. Combs, and Ahmet Yildiz. (2012) Cytoplasmic Dynein Moves Through Uncoordinated Stepping of the AAA+ Ring Domains. Science 335: 221.doi:10.1126/science.1215804.
Miraloma Tech, LLC
